When Marvel's Agents of S.H.I.E.L.D. debuted on ABC back in 2013, it was clearly supposed to be set within the Marvel Cinematic Universe, but as the show progressed, the divide between the big-screen franchise and Marvel Television deepened and the series pretty much became its own thing.
Even so, rumors have persisted that at least some Agents of S.H.I.E.L.D. characters (Chloe Bennet's Quake and Ming Na-Wen's Melinda May seem like the most popular possibilities) could show up in the MCU, and with the likes of Netflix's Daredevil and X-Men: The Animated Series getting new leases of life, there's speculation that more AOS could follow at some point.
Marvel's Head of Television, Animation, and Streaming Brad Winderbaum was asked about a potential revival during an interview with Agents of Fandom, and while he did sidestep the question somewhat, it sounds like there is a chance it could happen.
“We’re doing that right now. We’re in production on Daredevil: Born Again in New York, they’re shooting as we speak — I’m seeing text messages come up from set. That’s probably the next show that is going to kind of revisit and revive a really strong, and one of my favorite pockets of our universe and our fandom. And… you never know, is the answer!”
If AOS does return in some capacity, we can't see it being a full-on revival featuring all of the main players. But if there are plans to reintroduce any of the characters, the Multiverse-spanning events of the upcoming Avengers movies would seem like the ideal opportunity.
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"Marvel's Agents of S.H.I.E.L.D. is an American television series created by Joss Whedon, Jed Whedon, and Maurissa Tancharoen for ABC based on the Marvel Comics organization S.H.I.E.L.D. (Strategic Homeland Intervention, Enforcement, and Logistics Division), a peacekeeping and spy agency in a world of superheroes. The series was the first to be set in the Marvel Cinematic Universe (MCU), and it acknowledges the continuity of the franchise's films and other television series. It was produced by ABC Studios, Marvel Television, and Mutant Enemy Productions, with Jed Whedon, Maurissa Tancharoen, and Jeffrey Bell serving as showrunners.
The series stars Clark Gregg as Phil Coulson, reprising his role from the film series, alongside Ming-Na Wen, Brett Dalton, Chloe Bennet, Iain De Caestecker, and Elizabeth Henstridge. Nick Blood, Adrianne Palicki, Henry Simmons, Luke Mitchell, John Hannah, Natalia Cordova-Buckley, and Jeff Ward joined in later seasons. The S.H.I.E.L.D. agents deal with various unusual cases and enemies, including Hydra, Inhumans, Life Model Decoys, alien species such as the Kree and Chronicoms, and time travel. Several episodes directly cross over with MCU films or other television series, notably Captain America: The Winter Soldier (2014), which significantly affected the series in its first season, and Agent Carter (2015–16), from which series regular Enver Gjokaj joined the cast for the seventh season. In addition to Gregg, other actors from throughout the MCU also appear in guest roles."